Molecule Immune

Molecule Immune

Molecular Immunotechnology
WHO-sponsored Filarial Genome project

Prof. Kaliraj's group was involved in sequencing a total of 17,000 ESTs comprising of 7000 genes of B.malayi. Functional genomic analysis revealed that 30% of the genes were unique for filarial parasite, 27% being structural genes and 20% being enzymes. Genes such as SXP-1, Abundant Larval Transcript 2(ALT), Translationally Controlled Tumor Protein (TCTP), VAH, Thioredoxin, Transglutaminase, Thioredoxin peroxidase, MIF-1, HSP70 and endochitinase from the filarial parasites, Brugia malayi and Wuchereria bancrofti, have been identified as candidates for diagnosis and prophylactic measures. The immunodiagnostic and immunoprophylactic applications using these parasitic gene products have been extensively studied.


Immuno diagnosis of human lymphatic filariasis

As a part of global effort to eliminate lymphatic filariasis, a major public health problem for India and many other countries in the world, highly sensitive and specific diagnostic tests are being developed for close monitoring and evaluation of the control programs.Newer diagnostic tools reported based on antigen detection (Og4C3 and ICT card test kits) are limited by their ability to detect only bancroftian infection and not brugian infections. It is also essential to develop a diagnostic kit to identify prevalence of mixed filarial infection due to W. Bancrofti and B.malayi.

Prof. Kaliraj’s group has identified WbSXP-1 as a promising and a more reliable candidate for the identification of active infections (Microfileamics) in both bancroftian and brugian filariasis. Simple and rapid qualitative Immunodiagnostic test kits for the identification of antigen (monoclonal antibody to WbSXP-1 based) and antibody (recombinant filarial antigen WbSXP-1 based) from individuals with W.bancrofti/ B.malayi/mixed infections by Flow-through Assay (antibody test) and Immuno-chromatographic card test (antigen test) have been developed in collaboration with SPAN Diagnostics, Surat, P.A.R.I.S, Immunologicals, and UTC, Compiegne France. This product is currently under field evaluation for commercialization. Confidentiality and Material Transfer agreements have been executed by Prof. Kaliraj with M/S Span Diagnostics Surat for Manufacturing and Marketing in India and with M/S PANBIO Australia for Global marketing of these kits. This (is being worked out) / (serves) as a successful model for commercialization of a research product involving university, national and international commercial and noncommercial organizations / partners.

In the next phase stage-specific detection kits are being developed using recombinant DNA Technology and monoclonal antibodies for effective control of the disease. Compact Disc based high throughput immunoassays In many immunoassays like ELISA antigen or antibody is adsorbed onto the plastic surface of a microtitre plate. In collaboration with Glasgow university, UK., University of Heidelberg, Germany and Aims Sham's University, Egypt under EC project Prof. Kaliraj and his group have designed a compact disc (CD) platform which can replace the microtitre plate for antigen capture immunoassays as a cost effective and versatile method for mass diagnosis.

Immunoprophylaxis

The need for a lymphatic filariasis vaccine for complete eradication of the disease is imperative. Prof. Kaliraj's work carried out at the Centre in the past 10 years in collaboration with NIH, USA; Fermentile Hospital, Australia and JBTDRC, Wardha has resulted in the identification of novel stage-specific antigen ALT2 and other targets like VAH, transglutaminase, SXP, endochitinase, TCTP, thioredoxin, etc. as potential vaccine candidates. The results of the immunoprophylactic studies in mouse and gerbil models using ALT2 as a protein vaccine as well as a DNA vaccine are promising. Three putative vaccine candidates having partial lengths of ALT2, VAH and Thioredoxin peroxidase genes have been identified by screening the T7 phage

CENTRE FOR BIOTECHNOLOGY & FOOD TECHNOLOGY

Centre for Biotechnology

The Centre for Biotechnology was established in 1987 in Anna University with financial support from Department of Biotechnology, Delhi, University Grants Commission, Delhi and Anna University with the following objectives:

To carry out fundamental research in the frontier areas of biotechnology
To promote research and consultancy activities in various areas of biotechnology.
To enhance the human resource in Biotechnology and Food technology through training of students towards Ph.D programme
The Centre has a well defined building at Taramani campus with facilities for carrying out research in various areas of Biotechnology, housing modern infrastructure to conduct work in Bioprocess Technology, Immunology, Drug Discovery, Chemical Biology, Computational Biology, Stem Cell Biology.

Apart from the research centre, in the main campus of Anna University the Centre has an Administrative building and Teaching complex.

Several laboratory findings have been published in reputed international journals and presented at many national and international conferences. The UGC has recognized the Department under Department Research Scheme. The Department of Biotechnology, Government of India has granted a long term Program Support in the areas of:

Diagnostic Platform for Infectious Diseases
Macromolecular structure and Design
The areas of research are contemporary and the Centres boast of a good mix of experienced researchers and vibrant young faculty.

Centre for Food Technology


The Centre for Food Technology was established in 2010 to meet the requirements for well qualified and trained human resources to cater to the needs of the rapidly growing food processing sector. A grant for a period of five years had been sanctioned by Department of Biotechnology, Government of India to Anna university, Chennai, to establish the Centre with the objectives:
To carry out research in the frontier areas of Food Process and Nutrition
To develop manpower in these areas
The Centre provides various laboratory facilities to process food products for practical as well as research purpose. The Centre maintains links with various research institutes and food processing industries and also provides consultancy services to various reputed organizations.

Before we enter into anything ,we just want to know about its applications & merits & demerits.
Thus, hereby we are going to discuss about the applications of biotechnological science.

Applications Of Biotechnology

Top Biotechnology Schools and Colleges in USA

Biotechnology has application in four major industrial areas, including health care (medical), crop production and agriculture, non food (industrial) uses of crops and other products (e.g. biodegradable plastics, vegetable oil, biofuels), and environmental uses.

Applications of BIOTECHNOLOGY in Medicine

Biotechnology is a very huge field and its applications are used in a variety of fields of science such as agriculture and medicine. Medicine is by means of biotechnology techniques so much in diagnosing and treating dissimilar diseases.... Read More

Applications of Biotechnology in Agriculture

Biotechnology is regularly deliberated the same with the biomedical research, but there are a lot of other industries which take benefit of biotech method for studying, cloning and varying genes. We have become recognizable to the thought of enzymes in our everyday lives and many people are recognizable with the argument adjacent the use of GMOs in our foods.

Applications in Food and Processing

Application of biotechnology to food processing in developing countries is an issue of debate and discussions for a long time. Biotechnological study as practical to bioprocessing in the bulk of rising countries, targets development and improvement of customary fermentation processes. However there are a few issues which need to be discussed in developing countries while using the technology for various applications..... Read More

Applications of Biotechnology in Environmental

The application of Biotechnology to solve the environmental problems in the environment and in the ecosystems is called Environmental Biotechnology. It is applied and it is used to study the natural environment. According to the international Society for environmental Biotechnology the environmental Biotechnology is defined as an environment that helps to develop, efficiently use and regulate the biological systems and prevent the environment from pollution or from contamination of land, air and water have work efficiently to sustain an environment â€" friendly Society...... 

BIOTECHNOLOGY:Introduction

Biotechnology

Biotechnology , which is the basic life science that makes the use of an organism to promote and manage the life .

In short, Biotechnology is the one of the science technologies that create and destroy an organism.

For thousands of years, humankind has used biotechnology in agriculture, food production, and medicine.^[2] 

The term is largely believed to have been coined in 1919 by Hungarian engineer Károly Ereky. 

In the late 20th and early 21st century, biotechnology has expanded to include new and diverse sciences such as genomics, recombinant genetechniques, applied immunology, and development of pharmaceuticaltherapies and diagnostic tests.^[2]

The BIOTECH is a combination of BIOLOGY & BIOCHEMISTRY.

Biology

"Biological science" redirects here. It is not to be confused with life science.

For other uses, see Biology (disambiguation).

Biology deals with the study of the many living organisms. (top: E. coli bacteria and gazelle) (bottom: Goliath beetle and tree fern)

Biochemistry

Biochemistry, sometimes calledbiological chemistry, is the study ofchemical processes within and relating to living organisms.^[1] By controlling information flow through biochemical signaling and the flow of chemical energy through metabolism, biochemical processes give rise to the complexity of life. Over the last decades of the 20th century, biochemistry has become so successful at explaining living processes that now almost all areas of the life sciences from botany tomedicine to genetics are engaged in biochemical research.^[2] Today, the main focus of pure biochemistry is in understanding how biological molecules give rise to the processes that occur within living cells, which in turn relates greatly to the study and understanding of whole organisms.

Biochemistry is closely related tomolecular biology, the study of the molecular mechanisms by whichgenetic information encoded in DNA is able to result in the processes of life. Depending on the exact definition of the terms used, molecular biology can be thought of as a branch of biochemistry, or biochemistry as a tool with which to investigate and study molecular biology.

Much of biochemistry deals with the structures, functions and interactions of biological macromolecules, such asproteins, nucleic acids, carbohydratesand lipids, which provide the structure of cells and perform many of the functions associated with life. The chemistry of the cell also depends on the reactions of smaller molecules andions. These can be inorganic, for example water and metal ions, ororganic, for example the amino acidswhich are used to synthesize proteins. The mechanisms by which cells harness energy from their environment via chemical reactions are known asmetabolism. The findings of biochemistry are applied primarily in medicine, nutrition, and agriculture. In medicine, biochemists investigate the causes and cures of disease. In nutrition, they study how to maintain health and study the effects of nutritional deficiencies. In agriculture, biochemists investigate soil and fertilizers, and try to discover ways to improve crop cultivation, crop storage and pest control.